Nitro Blue Tetrazolium Chloride monohydrate
Nitro Blue Tetrazolium Chloride monohydrate
CAS: 298-83-9
Molecular Formula: C40H30ClN10O6+
Nitro Blue Tetrazolium Chloride monohydrate - Names and Identifiers
| Name | Nitro Blue Tetrazolium Chloride monohydrate |
| Synonyms | NBT Nitro BT nbt(dye) nitrotetrazoliumbt tetrazoliumnitrobt tetrazoliumnitroblue Nitrotetrazoliumblau nitrobluetetrazoliumsalt nitrotetrazoliumchlorideblue Nitrotetragolium blue chloride Nitrotetrazolium Blue chloride Nitro Blue Tetrazolium Chloride Nitrotetrazolium Blue chloride monohydrate Nitro Blue Tetrazolium Chloride monohydrate 2h-tetrazolium,3,3'-(3,3'-dimethoxy(1,1'-biphenyl)-4,4'-diyl)bis(2-(4-nitrophe 2h-tetrazolium,3,3'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[2-(4-nitrophe 2h-tetrazolium,3,3'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[2-(4-nitrophe 3,3'-(3,3'-dimethoxybiphenyl-4,4'-diyl)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium] 5,5-diphenyl-3,3-bis(4-nitrophenyl)-2,2-(3,3-dimethoxybiphenyl-4,4-ylene)ditetrazolium dichloride 3,3'-(3,3'-dimethoxybiphenyl-4,4'-diyl)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium] dichloride 2,2'-(3,3'-dimethoxybiphenyl-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-2,3-dihydro-1H-tetrazol-3-ium] dichloride 2-[2-methoxy-4-[3-methoxy-4-[3-(4-nitrophenyl)-5-phenyl-2-tetrazolidinyl]phenyl]phenyl]-3-(4-nitrophenyl)-5-phenyltetrazolidine |
| CAS | 298-83-9 |
| EINECS | 206-067-4 |
| InChI | InChI=1/C40H32N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3,(H,41,43)(H,42,44);2*1H |
Nitro Blue Tetrazolium Chloride monohydrate - Physico-chemical Properties
| Molecular Formula | C40H30ClN10O6+ |
| Molar Mass | 782.19 |
| Density | 1.5521 (rough estimate) |
| Melting Point | 200°C |
| Water Solubility | Soluble in water, ethanol and methanol. |
| Solubility | Soluble in water and ethanol, slightly soluble in ethyl acetate, insoluble in ether |
| Appearance | Light yellow powder or yellow needle crystal |
| Color | Yellow |
| Maximum wavelength(λmax) | ['256 nm'] |
| BRN | 4115923 |
| Storage Condition | 2-8°C |
| Stability | Stable, but store cold. |
| Sensitive | Light Sensitive |
| Refractive Index | 1.7350 (estimate) |
| MDL | MFCD00012159 |
| Use | This product is for scientific research only and shall not be used for other purposes. |
Nitro Blue Tetrazolium Chloride monohydrate - Risk and Safety
| Risk Codes | R22 - Harmful if swallowed R68/20/21/22 - R20/21/22 - Harmful by inhalation, in contact with skin and if swallowed. R36 - Irritating to the eyes R36/37/38 - Irritating to eyes, respiratory system and skin. R41 - Risk of serious damage to eyes |
| Safety Description | S36 - Wear suitable protective clothing. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) S36/37 - Wear suitable protective clothing and gloves. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S39 - Wear eye / face protection. |
| WGK Germany | 3 |
| RTECS | XF8045000 |
| FLUKA BRAND F CODES | 8-10-23 |
| TSCA | Yes |
| HS Code | 32041900 |
| Toxicity | LD50 orl-mus: 2 g/kg YAKUD5 32,614,1990 |
Nitro Blue Tetrazolium Chloride monohydrate - Reference
| Reference Show more | 1. Fu Li, Yue, happy. Effect of new liquid infant formula on immune function in mice [J]. Science and Technology of food industry, 2017(04):357-360. 2. Ding Junxi, Zhang Chaohua, Gao Jialong, et al. Immunomodulatory effects of ultrafiltration components from enzymatic hydrolysis products of isobarth clam meat [J]. Journal of Guangdong Ocean University, 2020, v.40(03):118-125. 3. Zhang Zhiwei, Li Yueyue, He Cucu, etc. Active oxygen content and metabolic enzyme activities of Ganoderma lucidum fruiting bodies under hyperoxia [J]. Chinese Journal of Bacteriology, 2020, 039(001):99-109. 4. Chen richun, Xiong Wenfei, Cai Yinan, etc. Antioxidant activity of collagen peptides from silver carp scales [J]. Journal of Food Science and Technology, 2013, 31(006):28-35. 5. Xu Yaqin, Fu xuetin, Wang Libo, etc. Effects of acetylation modification on structural characteristics and activity of polysaccharides from black currant fruit [J]. Fine Chemicals, 2019, 36(12). 6. Qiu Zhen, Yu, fan, Li, Guo, Zhi, et al. Effects of sweating in different environments on the content of effective components and antioxidant activity of Salvia miltiorrhiza Bunge [J]. Natural product research and development 2017(10):82-86 114. 7. Wang, Hanbo, et al. "Methyl jasmonate has been induced by the resistance of postharvest blue to gray mold cast by Botrytis cinerea." Journal of the Science of Food and Agriculture 100.11 (2020): 4272-4281.https:// doi.org/10.1002/jsfa.10469 8. Li, Guozhuan, et al. "Comparison of the chromatographic finger print, multicomponent quantitation and antioxidant activity of Salvia miltiorrhiza Bge. between sweating and nonsweating." Biomedical Chromatography 32.6 (2018): e4203.https://doi.org/10.1002/bm 9. [IF=9.381] Yi-Peng Bai et al."Effect of thermal processing on the molecular, structural, and antioxidant characteristics of highland barley β-glucan."Carbohyd Polym. 2021 Nov;271:118416 10. [IF=7.514] Li Zhao et al."Comprehensive analysis of the anti-glycation effect of peanut skin extract."Food Chem. 2021 Nov;362:130169 11. [IF=7.086] Tao Luo et al."Strengthening arsenite oxidation in water using metal-free ultrasonic activation of sulfite."Chemosphere. 2021 Oct;281:130860 12. [IF=6.312] Xiong Gao et al."Purification, Physicochemical Properties and Antioxidant Activities of Two Low-Molecular-Weight Polysaccharides from Ganoderma leucocontextum Fruiting Bodies."Antioxidants-Basel. 2021 Jul;10(7):1145 13. [IF=4.568] Lijun Xu et al."Amplified Peroxidase-like Activity of Co2 Using 8-Hydroxyquinoline and Its Application for Ultrasensitive Colorimetric Detection of Clioquinol."Chemistry-An Asian Journal. 2021 Oct 15 14. [IF=3.638] Hanbo Wang et al."Methyl jasmonate induces the resistance of postharvest blueberry to gray mold caused by Botrytis cinerea."J Sci Food Agr. 2020 Aug;100(11):4272-4281 15. [IF=6.057] Lijun Xu et al.Accelerating the peroxidase-like activity of Co2 by quinaldic acid: Mechanism and its analytical applications.Talanta. 2021 Nov;:123080 16. [IF=6.953] Yi-Peng Bai et al."Impact of thermally induced wall breakage on the structural properties of water-soluble polysaccharides in chickpeas."Int J Biol Macromol. 2022 May;208:869 17. [IF=6.475] Li Zhao et al."Effects of A- type oligomer procyanidins on protein glycation using two glycation models coupled with spectroscopy, chromatography, and molecular docking."Food Res Int. 2022 May;155:111068 |
Nitro Blue Tetrazolium Chloride monohydrate - Introduction
Substrates for dehydrogenase and other oxidases. For the determination of glucose -6-phosphate dehydrogenase. It is also a precipitation reagent for coagulation factor I(fibrinogen). It is also one of the alkaline phosphatase substrates, which generates insoluble blue products under the catalysis of alkaline phosphatase. It is commonly used in immunohistochemistry (IHC), immunocytochemistry (ICC) and Western blot (WB) experiments. Quantification of oxidoreductase and steroids. Assay of succinate dehydrogenase in histochemistry.
Last Update：2022-10-16 17:24:22
Nitro Blue Tetrazolium Chloride monohydrate - Reference Information
| biological field application | Diagnosis of Alzheimer's disease,bacterial vaginosis,behavioral disturbances in children,Hirschsprung disease; phosphatase assay; phytase assay; detecting bacteria,microorganisms,phosphoinositides, east; generating and detecting |
| EPA chemical information | Information provided by: ofmpub.epa.gov (external link) |
| Biological activity | Nitro blue tetrazolium chloride (NBT) is a dehydrogenase substrate, used together with alkaline phosphatase substrate BCIP for western blotting and immunohistochemical staining. |
| Uses | Substrate for dehydrogenase or oxidase, often combined with BCIP, used to detect peralkaline phosphoesterase activity Substrate for dehydrogenase and other peroxidase. Used for the determination of glucose-6-phosphate dehydrogenase (glucose-6-phosphate dehydrogenase); It is also a precipitation reagent for coagulation factor I(fibrinogen). It is also one of the alkaline phosphatase substrates, which generates insoluble blue products under the catalysis of alkaline phosphatase. Commonly used in immunohistochemistry (IHC), immunocytochemistry (ICC) and Western blot (WB) experiments. |
Last Update：2024-04-10 22:29:15
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